Question 1

Which of the following observations was the MOST crucial in the development of recombinant baculoviruses that can express large amounts of foreign proteins?

Accepted Answer

A)  Baculoviruses can enter mammalian cells but can not replicate.
B)  The polyhedrin gene is not essential for virus replication in cell culture.
C)  Viruses missing the polyhedrin gene can be distinguished from wild-type viruses microscopically.
D)  Recombinant viruses can produce large amounts of a foreign protein when introduced into insect cells in culture.
E)  The polyhedrin gene has a very strong promoter that produces large amounts of mRNA late in the infection.F) A) and E)
G) A) and C)

Question 2

How do the hosts of baculoviruses typically become infected with the virus?

Accepted Answer

A)  They are injected with virus by a parasitic wasp
B)  They hatch from an infected egg.
C)  They breathe in aerosoled virus particles.
D)  They feed on virus contaminated plants.
E)  They mate with an infected host.F) C) and D)
G) C) and E)

Question 3

Treatment of cells infected with baculovirus with $\alpha$-amanitin toxin will inhibit immediate-early or early but not late viral gene expression.

Accepted Answer

A) True 
 B)False

Question 4

The occlusion bodies of baculovirus consist of a single protein.Which of the following is this protein?

Accepted Answer

A)  polyhedrin
B)  occludin
C)  tegument
D)  GP64
E)  baculinF) B) and D)
G) A) and D)

Question 5

Which of the following describes the size and structure of the dsDNA genome of AcMNPV,a member of the baculovirus family?

Accepted Answer

A)  Covalently closed circle of about 50kbp.
B)  Covalently closed circle of about 150kbp.
C)  Linear molecule of about 50kbp.
D)  Linear molecule of about 150kbp.
E)  Three linear segments of about 50kbp each.F) D) and E)
G) C) and D)

Exam 25: Baculoviruses

How do the hosts of baculoviruses typically become infected with the virus?

Correct Answer
verified

The occlusion bodies of baculovirus consist of a single protein.Which of the following is this protein?

Correct Answer
verified

Which of the following observations was the MOST crucial in the development of recombinant baculoviruses that can express large amounts of foreign proteins?

Correct Answer
verified

Which of the following describes the size and structure of the dsDNA genome of AcMNPV,a member of the baculovirus family?

Correct Answer
verified

Treatment of cells infected with baculovirus with $\alpha$ -amanitin toxin will inhibit immediate-early or early but not late viral gene expression.

Correct Answer
verified

Exam 25: Baculoviruses

How do the hosts of baculoviruses typically become infected with the virus?

Correct AnswerverifiedShow Answer

The occlusion bodies of baculovirus consist of a single protein.Which of the following is this protein?

Correct AnswerverifiedShow Answer

Which of the following observations was the MOST crucial in the development of recombinant baculoviruses that can express large amounts of foreign proteins?

Correct AnswerverifiedShow Answer

Which of the following describes the size and structure of the dsDNA genome of AcMNPV,a member of the baculovirus family?

Correct AnswerverifiedShow Answer

Treatment of cells infected with baculovirus with α\alphaα -amanitin toxin will inhibit immediate-early or early but not late viral gene expression.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Treatment of cells infected with baculovirus with $\alpha$ -amanitin toxin will inhibit immediate-early or early but not late viral gene expression.

Correct Answer
verified